Working with other collaborators at Tulane University and USAMRIID, the Sabeti lab has obtained a vast repository of valuable clinical samples from patients with viral hemorrhagic fevers. Our hope is to carry out in-depth genomic analysis and diversity studies, elucidating regions of selection and identifying novel strains. The ability to generate full-length sequences will help in creating accurate phylogenies for both Lassa fever and Ebola viruses, as well as refine our ability to predict and identify regions of selection and high mutation within the genomes of these viruses.
Using both 454 and Illumina technologies, the Sabeti lab is developing novel approaches to full-length genome sequencing for both Lassa fever virus and Ebola virus. With the capacity to generate millions of base pairs of data from less than a teaspoon of blood, we are able to assemble complete viral genomes for identification and genetic analysis of known and unknown viruses.